中文题名 / Chinese title: 核蛋白编码基因支持肺鱼而非腔棘鱼是陆生脊椎动物最近的现生亲属
英文题名 / English title: Nuclear protein-coding genes support lungfish and not the coelacanth as the closest living relatives of land vertebrates.
作者 / Authors: Brinkmann H, Venkatesh B, Brenner S, Meyer A.
期刊 / Journal: PNAS
年份 / Year: 2004
DOI: 10.1073/pnas.0400609101
论文链接 / Article: https://doi.org/10.1073/pnas.0400609101
摘要来源 / Abstract source: https://europepmc.org/article/MED/15037746

英文摘要原文 / Original English abstract
The colonization of land by tetrapod ancestors is one of the major questions in the evolution of vertebrates. Despite intense molecular phylogenetic research on this problem during the last 15 years, there is, until now, no statistically supported answer to the question of whether coelacanths or lungfish are the closest living relatives of tetrapods. We determined DNA sequences of the nuclear-encoded recombination activating genes (Rag1 and Rag2) from all three major lungfish groups, the Australian Neoceratodis forsteri, the South American Lepidosiren paradoxa and the African lungfish Protopterus dolloi, and the Indonesian coelacanth Latimeria menadoensis. Phylogenetic analyses of both the single gene and the concatenated data sets of RAG1 and RAG2 found that the lungfishes are the closest living relatives of the land vertebrates. These results are supported by high bootstrap values, Bayesian posterior probabilities, and likelihood ratio tests.

摘要依据的主要发现 / Findings based on the abstract
研究分别及联合分析澳洲、非洲、南美肺鱼与印尼腔棘鱼的 Rag1 和 Rag2 序列。多种统计方法一致支持肺鱼为陆生脊椎动物最近的现生姊妹群。
Rag1 and Rag2 sequences from Australian, African and South American lungfishes and the Indonesian coelacanth were analyzed separately and together. Multiple statistical approaches consistently recovered lungfishes as the living sister group of land vertebrates.

摘要依据的结论 / Conclusions based on the abstract
两个核基因支持肺鱼与四足动物的近缘关系，为登陆演化提供分子背景。
The two nuclear genes support a lungfish–tetrapod relationship, providing molecular context for the transition onto land.

可用全文文件 / Available full-text files